Recombinant Dna Technology- A Game Changer In Cancer Biology

Anecdotes in cancer biology revealing experimental strategies that revolutionized the field of oncology

What you'll learn
Different theories about cancer namely- chemical, chromosomal and viral, formed pieces of a puzzle that led to the birth of Oncogenes and Tumor suppressors
Explore how tumor viruses revealed a bigger picture of cancer and also how identification of some of the viruses contributed to rDNA technology
How cloning strategies accelerated the understanding of molecular dimension of carcinogenesis
Learn about the non-viral mechanism of tumorigenesis and the different mechanism of mutations occurring in cancer cells
Requirements
Students must be familiar with recombinant DNA tools and techniques and concepts of cloning
Students must know basic concepts and terms in molecular biology such as DNA, mRNA, chromosomes, mitosis, meiosis etc.
Description
This course on cancer biology  focuses on historical theories and  cloning strategies put forth to comprehend carcinogenesis. The objective is to travel back in time to the period when all the pieces of puzzle such as chemical carcinogens, retroviruses and chromosomal mutations came together to reveal the grand scheme of cancer and propose the underlying criteria for cancer development. The highlights of this lecture series are the experimental methods developed during 1960s to 1990s which built the foundation for the current  model of cancer which stems from two concepts namely- oncogenes and tumor suppressor genes.You'll learn about Ames test, pro-viral theory, the discovery of reverse transcriptase, Leukemogenesis theory, different mechanisms of tumor cell mutations occurring in the cells, knudson hypothesis and much more.You'll learn-Chemical theory section explains the formation of cancer. Somatic mutation hypothesis and Ames test are concepts covered under chemical theoryChromosomal theory section covers the experiments done to show that chromosomal aberrations cause cancer.Viral theory section deals with Rous sarcoma virus, pro-viral theory, the discovery of reverse transcriptase, Leukemogenesis theory.The experiments that indicated the presence of proto-oncogenes and different mechanisms of mutations occurring in cancer cells.If oncogenes are like accelerators in the car, then tumor suppressors are like brakes. Learn about the cell fusion experiment, Knudson hypothesis and loss of heterozygosity.How p53 (the guardian of the genome) and pRb tumor suppressors were discovered.As is with any course, this course is also backed by Udemy's 30 day money back guarantee.If you're not happy for any reason you will get a full refund.So take this course today, and get deeper insights in cancer biology.

Overview

Section 1: Introduction

Lecture 1 Introduction

Section 2: Chemical theory

Lecture 2 Somatic mutation hypothesis

Lecture 3 Ames test

Section 3: Chromosomal theory

Lecture 4 Experiments to identify chromosomal aberrations

Lecture 5 Experiments identifying DNA, mRNA and protein

Section 4: Viral theory

Lecture 6 Peyton Rous- RSV and Viral theory

Section 5: Tumor viruses and their role in Cancer research

Lecture 7 RSV infection: fate of cells and influence on tumor formation

Lecture 8 Proviral theory

Lecture 9 Reverse transcriptase and Integrase

Lecture 10 RSV experiments- Quiz

Section 6: Hunt for proto-oncogenes

Lecture 11 Varmus and Bishop experiment

Lecture 12 Src Kinase and search for other oncogenes

Lecture 13 leukemogenesis theory

Lecture 14 Hunt for proto-oncogenes - quiz

Section 7: Non-viral mehanism of tumorigenesis

Lecture 15 DNA transfection experiment

Lecture 16 Are viral and non-viral mechanisms different?

Lecture 17 Different mechanism of mutations for oncogenes

Lecture 18 Non-viral mehanism of tumorigenesis- Quiz

Section 8: Discovery of tumor suppressor genes

Lecture 19 Cell fusion experiment

Lecture 20 Knudson hypothesis

Lecture 21 Loss of heterozygosity

Lecture 22 Identification of pRB gene

Lecture 23 Identification of p53 gene

Lecture 24 Tumor suppressors

Section 9: Wrap Up

Lecture 25 Conclusion

Lecture 26 BONUS

High school students, college freshers and research students who are inquisitive enough to dig deeper into the powerful observations, brilliant experimental strategies and different theories about cancer that shaped the field of cancer biology we know today,This course is not for absolute beginners who are looking for a typical in-depth college curriculum and not for experts in the field who are looking for advanced knowledge.,This course would aid research students in tapping into the collective historical wisdom of the pioneers and use them as a guide in further advancing this field.