Isolation Of Dna And Rna Concepts

Isolation of Nucleic acids

What you'll learn
Introduction
Isolation of RNA
Isolation of DNA
Isolation Nucleic acids Applications
Isolation of DNA and RNA steps
Diagram support explanation
Cosmids for further learning
Requirements
Any life science student
Description
·Introduction:Step 1: Sample Collection and ProtectionStep 2: RNA PreparationStep 3: Quantitation of Isolated RNAStep 4: Storage of Isolated RNAStep 1: Sample Collection and ProtectionFinding the most appropriate method of cell or tissue disruption for your specific starting material is important for maximizing the yield and quality of your RNA preparation. During sample disruption for RNA isolation, Samples stored at 4°C generate intact RNA, even after storage for a month.Step 2: RNA PreparationA number of RNA preparation technologies are widely available that can be classified into four general techniques: organic extraction methods, spin basket formats, magnetic particle methods, and direct lysis methods. While all can be used to prepare high-quality RNA suitable for a wide variety of analysis techniques, there are several factors to consider in selecting the right purification technology.Direct Lysis MethodsDirect lysis methods perform sample preparation (not purification) by utilizing lysis buffer formulations that disrupt samples, stabilize nucleic acids, and are compatible with downstream analysis. Typically, a sample is mixed with lysis agent, incubated for some amount of time under specified conditions, and then used directly for downstream analysis. If desired, samples can often be purified from stabilized lysates. By eliminating the need to bind and elute from solid surfaces, direct lysis methods can avoid bias and recovery efficiency effects that may occur when using other purification methods.

Overview

Section 1: Introduction

Lecture 1 Introduction

Lecture 2 Isolation of DNA part 1

Lecture 3 Isolation of DNA part 2

Lecture 4 Isolation of DNA and applications

Lecture 5 Isolation of RNA part 1

Lecture 6 Isolation of RNA part 2

Lecture 7 Isolation of RNA last part

Lecture 8 Additional Learning

All life science Msc students,Bsc students,Bio technology,Bio chemistry,Medical student,Pharmacy,Pharmacy