Elisa Principals And Applications For Professionals

ELISA fundementals

What you'll learn
Basic immunology
Monoclonal antibody
different types of antigens
different methods for antigen detection
common diagnostic techniques
enzyme immunoassay
mycotoxin and mycotoxicosis
food hormones residues

Requirements
basic knowledge of immunology

Description
course provides comprehensive training on the ELISA (Enzyme-Linked Immunosorbent Assay) technique, equipping professionals with the knowledge and skills for real-world applications.Key Topics:ELISA Fundamentals:Theoretical underpinnings of antigen-antibody interactionsVarious types of ELISA assays (direct, indirect, sandwich)Components and functions of ELISA kitsAdvanced Techniques:Double antibody sandwich ELISA for enhanced specificityCompetitive ELISA for quantifying small moleculesUnderstanding factors affecting ELISA performance (sensitivity, specificity)Applications in Diverse Fields:Immunodiagnostics of infectious diseases (e.g., COVID-19 testing)Food safety analysis (detecting foodborne pathogens)Environmental monitoring (assessing pollutant levels)Allergen detection in food productsAutoimmune disease diagnosticsData Analysis and Interpretation:Principles of spectrophotometry and result interpretationStatistical analysis of ELISA dataQuality control and validation proceduresCourse Benefits:Gain a thorough understanding of ELISA principles and methodologies.Master various ELISA applications relevant to your professional field.Develop critical skills in data analysis and interpretation of ELISA results.Become proficient in performing and troubleshooting ELISA assays.Enhance your problem-solving abilities for diverse applications.Target Audience:This course is designed for professionals from various backgrounds seeking to expand their knowledge and expertise in ELISA techniques. It is ideal for:Researchers in immunology, microbiology, and biotechnologyLaboratory technicians and analysts in food safety, environmental, and clinical settingsQuality control personnel in the pharmaceutical and food industriesMedical professionals seeking a deeper understanding of diagnostic toolsPrerequisites:A basic understanding of biological principles (antigens, antibodies, immune system) is recommended.Enzyme-linked immunosorbent Assay  (ELISA)        ELISA from fundamentals of immune system and the history of theanalytical assay prior to the invention of ELISA to the materials of choice forfabrication of the platforms, possible biomolecular interactions, different protocols,and evaluation parameters among the rest. This book guides Readers through different steps of the analytical assay while familiarizing them with the possiblesources of error in the assay. The course offers detailed insights on the immobilization techniques used for protein attachment, different methods for evaluationof the assay and calculation of the key important parameters in the analytical assaysuch as sensitivity, specificity, accuracy, and limit of detection. Advantages andshortages of the conventional ELISA as well as different attempts for improvementof its performance are covered in this book. Merging and intergrading differenttechnologies with widely known ELISA have opened numerous windows ofopportunity to the advancement of this immunoassay. In that respect, the currentbook provides the latest updates on integrated platforms such as ELISpot, plasmonic ELISA, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs as well asELISA in micro-devices. Fundamentals and History of ELISA Evolution of the Immunoassays Until Invention of ELISA Principles of the Immune System Biomolecular Interactions Between Antibody and Antigen . . .General Overviews on Applications of ELISA . . .Step by Step with ELISA: Mechanism of Operation, CrucialElements, Different Protocols, and Insights on Immobilizationand Detection of Various Biomolecular Entities . . . . . . . . . Evaluation of the Detection Results Obtained from ELISA . . . .

Who this course is for:
for lab professionals ,lab technicians, students in lab technology field