Biotechnology: Gene Editing Techniques

Learn about gene editing techniques like ZFNs, TALEN, and CRISPR

What you'll learn
What is Gene Editing?
History of Gene Editing
How Does Gene Editing Work?
Homologous Recombination
Peptide Nucleic Acids (PNAs)
Meganuclease
Zinc-finger nuclease (ZFNs)
Transcription activator-like effector nucleases (TALEN)
General Mechanism of CRISPR/Cas
Types I CRISPR/Cas System
Types II CRISPR/Cas System
Types III CRISPR/Cas System
Ethical Concerns Related to Gene Editing
Applications of Gene Editing
Requirements
Basic knowledge of biology, Biotechnology, and molecular biology
Interested in learning latest techniques
Motivation to Learn
Description
Over the last half century after post-DNA helical structure discovery, the world has seen a continuous staircase outburst of various molecular technologies, which are now heading forward toward translation into clinical and laboratory practice. Given the availability of sequencing platforms, acquired wisdom about the micro-mechanics at work within the genetic apparatus, and the introduction of user-friendly nanotechnologies, it was possible for next-generation scientists to manipulate the genetic codes at various levels.Principally, gene editing techniques can be interpreted as methods where DNA sequences are changed by deletions, mRNA processing, and post-transcriptional modifications to result in altered gene expression, leading to functional behavior of proteinsGene editing is performed using enzymes, particularly nucleases that have been engineered to target a specific DNA sequence, where they introduce cuts into the DNA strands, enabling the removal of existing DNA and the insertion of replacement DNA.  The enormous knowledge and ongoing research have now been able to demonstrate methodologies that can alter DNA coding. The gene editing techniques  evolved from the earlier attempts like nuclease technologies, homologous recombination, and certain chemical methods (peptide nucleic acid). Molecular techniques like meganuclease, transcription activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs) initially emerged as genome-editing technologies.These initial technologies suffer from lower specificity due to their off-targets side effects. Moreover, from biotechnology’s perspective, the main obstacle was to develop simple but effective delivery methods for host cell entry. The latest discovery of CRISPR/Cas9 technology seems more encouraging by providing better efficiency, feasibility, and multi-role clinical application. The significant leap in gene editing techniques brought new urgency to long standing discussions about the ethical concerns surrounding the genetic engineering. Many questions, such as whether genetic engineering should be used to treat human disease or to alter traits such as beauty or intelligence, had been asked in one form or another for decades. This course generally discusses the various gene editing techniques in terms of the mechanisms of action, advantages, and side effects.This course is a valuable resource for students and researchers related to molecular biology, forensic science, medical laboratory technology, biotechnology, and genetics. Start your learning journey now and explore the hidden truth about nature!

Overview

Section 1: Overview

Lecture 1 Overview

Section 2: Gene Editing

Lecture 2 What is Gene Editing?

Lecture 3 History of Gene Editing

Lecture 4 How Does Gene Editing Work?

Section 3: Gene Editing Techniques

Lecture 5 Gene Editing Techniques

Lecture 6 Resources

Section 4: Conventional System

Lecture 7 Homologous Recombination

Lecture 8 Mechanism of Homologous Recombination

Lecture 9 Limitations Of Homologous Recombination

Section 5: Chemical System

Lecture 10 Peptide Nucleic Acids

Lecture 11 Mechanism and Limitations of Peptide Nucleic Acid

Section 6: Protein-Based Nuclease Systems

Lecture 12 Meganuclease

Lecture 13 Engineering of Meganuclease

Lecture 14 Mechanism of Meganuclease

Lecture 15 Zinc-Finger Nuclease

Lecture 16 Structure of Zinc-Finger Nuclease

Lecture 17 Mechanism of Zinc-Finger Nuclease

Lecture 18 Limitations and Strength of Zinc-Finger Nuclease

Lecture 19 TALEN

Lecture 20 Mechanism of TALEN

Lecture 21 Limitations and Strength of TALEN

Section 7: RNA Protein Based Systems

Lecture 22 CRISPR

Lecture 23 General Mechanism of CRISPR/Cas

Lecture 24 Types of CRISPR/Cas System

Lecture 25 Types II CRISPR/Cas System

Lecture 26 Types I CRISPR/Cas System

Lecture 27 Types III CRISPR/Cas System

Lecture 28 Limitations and Strength of CRISPR/Cas

Section 8: Ethical Concerns

Lecture 29 Ethical Concerns

Section 9: Applications of Gene Editing

Lecture 30 Applications of Gene Editing

Lecture 31 For further Reading

Students who want to go for lab technologist and molecular biologists job.,Researchers of molecular biology, biotechnology, and related disciplines.,Students who wants to persude their carrer in forensic science, medicine, research, molecular biology, and genetics.