Biochemistry: Learn Amino Acid And Protein Basics

Explore the properties of amino acids, protein folding, purification and their structure determination techniques

What you'll learn
Understand the properties of amino acids and their functions.
Know the principles of different protein purification techniques.
Learn about secondary and super-secondary structures
Get an understanding of the principle and working of different protein structure determination techniques
Learn the theories of protein folding and their experimental validation.
Requirements
To enroll in this course, all you need is some basic knowledge in chemistry.
Description
Proteins are the workhorses inside the cell that carryout various functions such as metabolic reactions, provide structure to cells, transport oxygen, antibodies that fight infections, hormones and much more. The main building blocks of proteins are amino acids and they constitute the primary sequence information of the protein. Understanding the properties of amino acids and how they are arranged in different types of proteins can tell us a lot about the function of the protein.In this course you’ll learn about1. The 3 types of classification of amino acids2. Learn how Ramachandran plot tells us about amino acid phi and psi angles that are involved in protein folding3. Learn the step-by-step process of protein sequencing4. Gain an understanding of the protein purification strategies5. How NMR and X-ray crystallography techniques are used to study the 3D structure of proteinsThis course contains has more than 2 hours of video content with 26 lectures explaining concepts pertaining to amino acids and proteins.By the end of the course, you’ll be able to· write the 3 and 1 letter code of amino acids,· Identify R group of different amino acids· Describe secondary and super secondary structures formed by proteins· Calculate pH, pKa and pI values of amino acids· Summarize the operating principles of X ray crystallography, NMR and circular dichroism· Explain the principles of different protein purification strategies· Describe protein folding theories that best explain protein folding inside the cells.This course also contains bonus pdf files of content and a quiz towards the end of the lecture series to reinforce your learning. Anyone who wishes explore more about amino acids and proteins will benefit from this course.This course is intended for purchase as an adult. Those under 18 may use this course only if a parent or guardian opens their account, handles any enrollments, and manages their account usage.

Overview

Section 1: Introduction

Lecture 1 Introduction

Section 2: Amino acid properites

Lecture 2 Basic properties

Lecture 3 Classification

Lecture 4 Modified amino acids

Lecture 5 Beta amino acids

Lecture 6 Information storage

Lecture 7 Ionization properties

Section 3: Secondary structure

Lecture 8 Ramachandran plot

Lecture 9 Secondary structures formed by amino acids

Lecture 10 Other secondary structures

Lecture 11 Super secondary structures

Section 4: Sequencing

Lecture 12 Protein sequencing- Edman degradation

Section 5: Protein Folding

Lecture 13 An analogy

Lecture 14 Levinthal's paradox

Lecture 15 Anfinsen's experiment

Lecture 16 Folding theories

Lecture 17 Experiments to determine folding

Lecture 18 Protein stability

Section 6: Protein purification

Lecture 19 Based on Solubility

Lecture 20 Based on Molecular weight

Lecture 21 Based on pI

Lecture 22 Based on specific activity

Lecture 23 Protein Yield

Section 7: Protein structure determination techniques

Lecture 24 Circular Dichroism

Lecture 25 Nuclear Magnetic resonance

Lecture 26 X ray crystallography

Section 8: Wrap Up

Lecture 27 Summary

Lecture 28 BONUS

This course is designed for college or high school students who want to learn the basics of protein biochemistry,This course is for those students who want to refresh their concepts on amino acids and proteins.