Become A Master In Molecular Biology

Learn DNA extraction, Electrophoresis, Blotting, Primer designing, PCR, DNA sequencing

What you'll learn
How DNA extraction is performed, components roles, what's the use of extracted DNA, DNA quantification
Electrophoresis, Agarose gel electrophoresis, Principle, How to make agarose gel, role of each component, DNA recovery
SDS- PAGE, role of stacking and seperating gel, role of each component, Buffer mechanism
Nucleic Acid Hybridization, Probing
Southern, Northern and Western blotting, their examples and applications, difference between different blotting techniques
PCR- Principle, procedure, analysis of PCR products, applications of PCR
Primers designing, parameters of primer designing, Tm- melting temperature
DNA sequencing- Difference between PCR and sequencing, detection, capillary electrophoresis and applications of DNA sequencing.
Requirements
Any biology student can learn
Description
The isolation of pure DNA is a first step in the progress of molecular studies in plant. Organic solvents such as chloroform, phenol or a mixture of phenol: chloroform: isoamyl alcohol (25:24:1) are used for denaturation and precipitation of proteins from nucleic acid solution and denatured proteins removed by centrifugation. The isolated DNA should be suitable for restriction digestion, amplification, cloning.To understand the basic techniques use to work with nucleic acids, remember that nucleic acids are macromolecules made up of nucleotides ( a sugar, phosphate group, nitrogenous base ).An entire set of DNA molecule in the nucleus of eukaryotic oraganism is called genome.DNA has complementary strands linked by hydrogen bonds between the paired bases.Agarose is a linear polymer extracted from seaweed.Purified agarose is a powder insoluble in water or buffer at room temperature but dissolves on boiling.Electrophoresis is a technique used to seperate charged molecules.The overall goal of course is to give student knowledge of molecular biology.More than 90% of content presented in short videos not longer than 10 minutes that concisely explains concepts.Pictures are attached to explore the concepts briefly.This course will be taught through ppt, pre- recorded lectures and quizzes.This course will taught with applications of real world.After completing this course you would be able to fully prepare for examination.

Overview

Section 1: Molecular Biology

Lecture 1 DNA extraction from plants

Lecture 2 Electrophoresis

Lecture 3 Quantification of DNA and applications

Lecture 4 Agarose gel electrophoresis

Lecture 5 SDS - PAGE

Lecture 6 Nucleic acid Hybridization

Lecture 7 Southern Blotting

Lecture 8 Northern Blotting

Lecture 9 Western blotting

Lecture 10 PCR

Lecture 11 Primer designing

Lecture 12 DNA sequencing

Lecture 13 Detection and applications of DNA sequencing

Lecture 14 Bonus lecture

Beginner to advanced