Mass Spectrometry of Protein Interactions

The authoritative guide to analyzing protein interactions by mass spectrometry Mass spectrometry (MS) is playing an increasingly important role in the study of protein interactions. Mass Spectrometry of Protein Interactionspresents timely and definitive discussions of the diverse range of approaches for studying protein interactions by mass spectrometry with an extensive set of references to the primary literature. Each chapter is written by authors or teams of authors who are international authorities in their fields. This leading reference text: * Discusses the direct detection of protein interactions through electrospray ionization (ESI-MS); ion mobility analysis; and matrix-assisted laser desorption/ionization (MALDI-MS) * Covers the indirect analysis of protein interactions through hydrogen-deuterium exchange (HX-MS); limited proteolysis; cross-linking; and radial probe (RP-MS) * Guides researchers in the use of mass spectrometry in structural biology, biochemistry, and protein science to map and define the huge number and diversity of protein interactions * Reviews the latest discoveries and applications and addresses new and ongoing challenges This is a comprehensive reference for researchers in academia and industry engaged in studies of protein interactions and an excellent text for graduate and postgraduate students.Content: Chapter 1 Direct Characterization of Protein Complexes by Electrospray Ionization Mass Spectrometry and Ion Mobility Analysis (pages 1–23): Joseph A. Loo and Catherine S. KaddisChapter 2 Softly, Softly—Detection of Protein Complexes by Matrix?Assisted Laser Desorption Ionization Mass Spectrometry (pages 25–43): Kevin M. DownardChapter 3 Probing Protein Interactions Using Hydrogen–Deuterium Exchange Mass Spectrometry (pages 45–61): David D. Weis, Suma Kaveti, Yan Wu and John R. EngenChapter 4 Limited Proteolysis Mass Spectrometry of Protein Complexes (pages 63–82): Maria Monti and Piero PucciChapter 5 Chemical Cross?Linking and Mass Spectrometry for Investigation of Protein–Protein Interactions (pages 83–107): Andrea SinzChapter 6 Genesis and Application of Radical Probe Mass Spectrometry (RP?MS) to Study Protein Interactions (pages 109–133): Simin D. Maleknia and Kevin M. Downard