Identification of Microorganisms by Mass Spectrometry, Volume 169

A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis From chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and cutting-edge mass spectrometry techniques for identifying microorganisms. A vital tool for microbiologists, health professionals, and analytical chemists, the text is designed to help scientists select the most effective techniques for use in biomedical, biochemical, pharmaceutical, and bioterror defense applications. Since microbiological applications of mass spectrometry require a basic understanding of both microbiology and analytical chemistry, the editors have incorporated material from both disciplines so that readers from either field will come to understand the necessary principles of the other. Featuring contributions from some of the most recognized experts in both fields, this volume provides specific examples of fundamental methods as well as approaches developed in the last decade, including: * Metastable atom bombardment pyrolysis mass spectrometry * Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) * MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria * High-resolution Fourier transform mass spectrometry (FTMS) * Electrospray ionization (ESI) mass spectrometry Identification of Microorganisms by Mass Spectrometry represents the most comprehensive and up-to-date work on the topic currently available. It is liberally illustrated with figures and tables and covers every aspect of spectrometric identification of microorganisms, including experimental procedures, various means of sample preparation, data analysis, and interpretation of complex mass spectral data.Content: Chapter 1 Cultural, Serological, and Genetic Methods for Identification of Bacteria (pages 1–21): John B. Sutherland and Fatemeh RafiiChapter 2 Mass Spectrometry: Identification and Biodetection, Lessons Learned and Future Developments (pages 23–37): Alvin FoxChapter 3 An Introduction to MALDI?TOF MS (pages 39–60): Rohana Liyanage and Jackson O. LayChapter 4 The Development of the Block II Chemical Biological Mass Spectrometer (pages 61–89): Wayne H. Griest and Stephen A. LammertChapter 5 Method Reproducibility and Spectral Library Assembly for Rapid Bacterial Characterization by Metastable Atom Bombardment Pyrolysis Mass Spectrometry (pages 91–124): Jon G. Wilkes, Gary Miertschin, Todd Eschler, Les Hosey, Fatemeh Rafii, Larry Rushing, Dan A. Buzatu and Michel J. BertrandChapter 6 MALDI?TOF Mass Spectrometry of Intact Bacteria (pages 125–152): Jackson O. Lay and Rohana LiyanageChapter 7 Development of Spectral Pattern?Matching Approaches to Matrix?Assisted Laser Desorption/Ionization Time?of?Flight Mass Spectrometry for Bacterial Identification (pages 153–160): Kristin H. Jarman and Karen L. WahlChapter 8 Studies of Malaria by Mass Spectrometry (pages 161–180): Plamen A. DemirevChapter 9 Bacterial Strain Differentiation by Mass Spectrometry (pages 181–201): Randy J. Arnold, Jonathan A. Karty and James P. ReillyChapter 10 Bacterial Protein Biomarker Discovery: A Focused Approach to Developing Molecular?Based Identification Systems (pages 203–227): Tracie L. Williams, Steven R. Monday and Steven M. MusserChapter 11 High?Throughput Microbial Characterizations Using Electrospray Ionization Mass Spectrometry and Its Role in Functional Genomics (pages 229–256): Seetharaman Vaidyanathan and Royston GoodacreChapter 12 Bioinformatics for Flexibility, Reliability, and Mixture Analysis of Intact Microorganisms (pages 257–277): Catherine Fenselau and Patrick PribilChapter 13 MALDI?FTMS of Whole?Cell Bacteria (pages 279–300): Jeffrey J. Jones, Michael J. Stump and Charles L. WilkinsChapter 14 A Review of Antibody Capture and Bacteriophage Amplification in Connection with the Direct Analysis of Whole?Cell Bacteria by MALDI?TOF MS (pages 301–318): Kent J. Voorhees and Jon C. ReesChapter 15 Discrimination and Identification of Microorganisms by Pyrolysis Mass Spectrometry: From Burning Ambitions to Cooling Embers—A Historical Perspective (pages 319–343): Eadaoin Timmins and Royston Goodacre