Quantitative PCR Protocols (Methods in Molecular Medicine) by Bernd Kochanowski
Humana Press; 1 edition | April 28, 1999 | English | ISBN: 0896035182 | 296 pages | PDF | 18 MB
Humana Press; 1 edition | April 28, 1999 | English | ISBN: 0896035182 | 296 pages | PDF | 18 MB
This is a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process.