Multiphoton Microscopy and Fluorescence Lifetime Imaging: Applications in Biology and Medicine
Karsten König, "Multiphoton Microscopy and Fluorescence Lifetime Imaging: Applications in Biology and Medicine"
English | ISBN: 3110438984 | 2018 | 450 pages | EPUB | 146 MB
English | ISBN: 3110438984 | 2018 | 450 pages | EPUB | 146 MB
This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard.
Contents
Part I: Basics
Brief history of fluorescence lifetime imaging
The long journey to the laser and its use for nonlinear optics
Advanced TCSPC-FLIM techniques
Ultrafast lasers in biophotonics
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