Proteomics in Practice: A Guide to Successful Experimental Design (2nd edition)
Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker - Proteomics in Practice: A Guide to Successful Experimental Design (2nd edition)
Published: 2008-05-05 | ISBN: 3527319417 | PDF | 502 pages | 6 MB
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.