The Extracellular Matrix
The Extracellular Matrix: Methods and Protocols
by Davide Vigetti
English | 2019 | ISBN: 1493991329 | 278 Pages | PDF | 7.42 MB
by Davide Vigetti
English | 2019 | ISBN: 1493991329 | 278 Pages | PDF | 7.42 MB
This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.