Bacteriophages: Methods and Protocols, Volume 3 (Methods in Molecular Biology)
Bacteriophages: Methods and Protocols, Volume 3 (Methods in Molecular Biology) by Martha R.J. Clokie
English | 16 Dec. 2017 | ISBN: 149397341X | 330 Pages | EPUB | 4.05 MB
English | 16 Dec. 2017 | ISBN: 149397341X | 330 Pages | EPUB | 4.05 MB
This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.