Proteomics for Drug Discovery: Methods and Protocols (Methods in Molecular Biology)
Proteomics for Drug Discovery: Methods and Protocols (Methods in Molecular Biology) by Iulia M. Lazar
English | 14 Sept. 2017 | ISBN: 1493972006 | 316 Pages | EPUB | 4.82 MB
English | 14 Sept. 2017 | ISBN: 1493972006 | 316 Pages | EPUB | 4.82 MB
This book reflects the drug development community’s interest in proteomics methods by providing researchers with the necessary knowledge for the implementation of a number of basic experimental and bioinformatics protocols in the drug discovery research laboratory. The focus of the collection is on select protocols and methodologies that address the analysis of post-translational modifications, targeted protein quantification, protein-protein, protein-lipid, or protein-ATP interactions, in silico lead identification, protein arrays, tissue and cell extract preparation, labeling, chemoproteomics, and drug efficacy assessment. Emphasis was placed not just on methods that describe large-scale protein analysis, but also on ones that are amenable to future implementation in a high-throughput format. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.