RNA Nanostructures: Methods and Protocols (Methods in Molecular Biology) by Eckart Bindewald
English | 6 Aug. 2017 | ISBN: 1493971379 | 400 Pages | EPUB | 5.69 MB
English | 6 Aug. 2017 | ISBN: 1493971379 | 400 Pages | EPUB | 5.69 MB
This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, RNA Nanostructures: Methods and Protocols is a valuable resource for the design and production of RNA nanostructures. Researchers and scientists sharing these detailed protocols is important for sustained progress in the field.