Circular RNAs (2nd Edition)
Circular RNAs
English | 2024 | ISBN: 1071636774 | 326 Pages | PDF (True) | 12 MB
English | 2024 | ISBN: 1071636774 | 326 Pages | PDF (True) | 12 MB
This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, in silico characterization, circRNA detection, sequence validation, quantification , techniques related to gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA translation potential, and vaccines based on circular RNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.