Mary F. Striegel, Jo Hill, "Thin-Layer Chromatography for Binding Media Analysis"

Foreword
Preface
Introduction
Part One: Handbook
Chapter 1: An Overview of Thin-layer Chromatography
Comparison of TLC to Other Chromatographic Methods
The History of TLC
The Early Years: 1938-1951
The Classical Period: 1956-1980
The Modern Period: 1981-Present
Theoretical Aspects of Thin-Layer Chromatography
Chapter 2: Technique of Thin-Layer Chromatography
Sample Preparation
Selection of the Chromatographic Plate
Selection of a Solvent System
Spotting the Sample
Development of the TLC Plate
Detection of Separation Zones
Visual Examination
Documentation
Chapter 3: Methodology for Thin-Layer Chromatography
Sorbent layers
Solvent Systems
Development Chambers
Detection Methods
Documentation
Chapter 4: Analysis of Proteins by Thin-Layer Chromatography
A Summary of the Use and Chemistry of Proteinaceous Binders
Analytical Methodology
Sample Preparation
Technique
Applications
Chapter 5: Analysis of Carbohydrates by Thin-Layer Chromatography
A Summary of the Use and Chemistry of Carbohydrate Binders
Analytical Methodology
Sample Preparation
Technique
Applications
Chapter 6: Analysis of Waxes by Thin-Layer Chromatography
A Summary of the Use and Chemistry of Wax Binders
Analytical Methodology
Sample Preparation
Technique
Applications
Chapter 7: Analysis of Resins by Thin-Layer Chromatography
A Summary of the Use and Chemistry of Resin Coatings
Analytical Methodology
Sample Preparation
Technique
Applications
Chapter 8: Visualization, Interpretation, Documentation, and Computer Analysis of Chromatographic Plates
Visualization Reagents
Physical Methods
Chemical Methods
Interpretation of the TLC Plate
Qualitative Methods
Quantitative Methods
Documentation
Manual-Graphical Methods
Photographic Methods
Written Record of Conditions
Computer Methods for Evaluation of the TLC Plate
Chapter 9: Scientific Examination of Works of Art
A Review of the Uses of TLC in the Examination of Works of Art
Protein Analysis
Carbohydrate Analysis
Wax Analysis
Resin Analysis
Some Potential Systems for Media Analysis by TLC
Part Two: Protocols
Introduction
Protocol A: Identification of Proteins by Thin-Layer Chromatography
Protocol B: Identification of Carbohydrates by Thin-Layer Chromatography
Protocol C: Identification of Waxes by Thin-Layer Chromatography
Protocol D: Identification of Resins by Thin-Layer Chromatography
Protocol E: Written Documentation of the TLC Plate
Protocol F: Photodocumentation of the TLC Plate Using Visible Light
Protocol G: Photodocumentation of the TLC Plate Using Ultraviolet Light
Protocol H: Sample Application for Thin-Layer Chromatography
Protocol I: Evaluation of a TLC Plate
Protocol J: Acid Hydrolysis in a Pierce Vial Reaction Chamber
Protocol K: Vapor Phase Acid Hydrolysis in a Pierce Vial Reaction Chamber
Protocol L: Semiquantitative Computer Analysis of a TLC Plate
Glossary
About the Authors

Mary F. Striegel was an assistant scientist at the Getty Conservation Institute for six years before joining the staff of the National Center for Preservation Technology and Training as a materials scientist in 1995. Her research includes the application of new analytical methods to the examination of artist materials. Her interests include technical photography and acoustic microscopy.

Jo Hill has specialties in objects conservation, textile conservation, and scientific analysis and research. She currently serves as director of conservation for the Fowler Museum of Cultural History. Her special interests include the materials and techniques of artifact fabrication, the conservation of painted surfaces, teaching, and the application of scientific research to the field of art conservation.