Laboratory Manual of Microbiology, Biochemistry and Molecular Biology P/B [Kindle Edition]

The applications of Microbiology, Biochemistry and Molecular Biology have led to the accumulation of a huge body of knowledge and phenomenal growth in procedures and methodologies. In recent years many novel methods have been developed and the old methods have been improved. Nevertheless the time tested classical techniques have been retained. The comprehensive practical training for any student studying biological sciences is must. Though many practical books are available in the market but this is an unique combination of protocols that covers maximum (about 80%) of the practicals of various Indian universities for UG and PG courses in Bioscience, Biotechnology, Microbiology, Biochemistry and Biochemical Engineering.

While preparing this laboratory manual the efforts have been made into various aspects of laboratory practices for the beginners like; the do’s and don’ts of working in any laboratory, concepts and terminologies used, and how to prepare the solutions/reagents. The protocols given here have been tested by authors during their long teaching experience. The book has been divided into four sections, the first one is Introduction which is subdivided into laboratory etiquette and safety, molecular, empirical and formula weight, planning a solution of a particular molarity, accuracy and calibration, buffers etc. Second part is about Instruments: Principle and Precautions which elaborates various commonly used equipments needed to perform different experiments. The third part of the book is about Experiments which has all the traditional to latest experiments with principle, requirements, procedure, results and observation and precautions in three major areas of Bioscience and Biotechnology i.e. Microbiology, Biochemistry and Molecular Biology. At the end a rather comprehensive Appendix is given as section four.

The microbiology section contains all the basic techniques used in laboratories and industries. It also consists of some advanced microbiological experiments related to industrial microbiology. The second section deals with the basic practicals associated with carbohydrates, lipids, amino acid analysis, and chromatographic techniques. Besides, other advanced techniques such as isoelectric focusing, SDS-PAGE etc. have also been included. As molecular biology has become an integral part of almost all biological courses, the third section of this book is dedicated to all the techniques related to and used in molecular biology. Some experiments related to mitochondrial and chloroplast DNA

x Laboratory Manual of Microbiology, Biochemistry & Molecular Biology
isolation, recombinant DNA transformation and selection, blotting and hybridization techniques which are generally not found in most practical books, have also been included here. All the three sections of the book i.e. microbiology, biochemistry and molecular biology have been written by authors who have long experience and are well versed with conducting practicals related to their expertise.

One person who has been instrumental for the initiation of this project and who has rendered his whole hearted support at all times for the successful completion of this book is none other than Dr. Ravi Israni. We owe him our heartfelt gratitude. The authors also wish to acknowledge the support and guidance provided by Prof. Aditya Shastri, Vice Chancellor and Prof. Vinay Sharma, Head, Dept. of Bioscience and Biotechnology of Banasthali University. For the preparation of this book we have consulted many practical books and we would like to extend our thanks to all the authors of these books namely K.R. Aneja, R.C. Dubey, D.K. Maheshwary, R.N. Bhattacharya, S. Sadasivam, A. Manickam etc.

Last but not least, we are greatly indebted to the almighty and our family members for their blessings and making our path smooth.

Jyoti Saxena Mamta Baunthiyal Indu Ravi