"Gel Electrophoresis: Principles and Basics" ed. by Sameh Magdeldin

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"Gel Electrophoresis: Principles and Basics" ed. by Sameh Magdeldin
InTeO | 2012 | ISBN: 9535104586 9789535104582 | 376 pages | PDF | 22 MB

In this book, the authors try to present simplified fundamentals of gel-based separation together with exemplarily applications of this versatile technique. The contents of the book crisp and comprehensive, it will receive overwhelming interest and deliver benefits and valuable information to the readers.

Contents
Part 1 Basic Principles of Gel Electrophoresis
1 Introduction to Agarose and Polyacrylamide Gel Electrophoresis Matrices with Respect to Their Detection Sensitivities
2 Gel-Electrophoresis and Its Applications
3 Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
4 Discriminatory Power of Agarose Gel Electrophoresis in DNA Fragments Analysis
5 Gel Electrophoresis of Proteins
6 Gel Electrophoresis of Protein - From Basic Science to Practical Approach
Part 2 Two Dimensional Polyacrylamide Gel Electrophoresis
7 Two-Dimensional Polyacrylamide Gel Electrophoresis - A Practical Perspective
8 High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis: ATool for Identification of Polymorphic and Modified Linker Histone Components
9 Two-Dimensional Gel Electrophoresis (2-DE)
10 High Speed Isoelectric Focusing of Proteins Enabling Rapid Two-Dimensional Gel Electrophoresis
Part 3 Denaturing Gradient Gel Electrophoresis (DGGE)
11 Denaturing Gradient Gel Electrophoresis (DGGE) in Microbial Ecology - Insights from Freshwaters
Part 4 Statistical and Bioinformatic Analysis of Electrophoresis Data
12 Statistical Analysis of Gel Electrophoresis Data
13 Quantitative Analysis of Electrophoresis Data - Application to Sequence-Specific Ultrasonic Cleavage of DNA
Part 5 Pulsed Field Gel Electrophoresis (PFGE)
14 The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Harmonization at the European Union Level
Part 6 Bacterial Electrophoretic Techniques
15 Electrophoretic Techniques in Microbial Ecology
16 Application of Multiplex PCR, Pulsed-Field Gel Electrophoresis (PFGE), and BOX-PCRfor Molecular Analysis of Enterococci
17 The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Comparison with MLVA Method Coupled with Gel Electrophoresis
18 Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments (PCR-RFLP) and Gel Electrophoresis - Valuable Tool for Genotyping and Genetic Fingerprinting
19 Application of Two-Dimensional Gel Electrophoresis to Microbial Systems

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