Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations, Fourth Edition

This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.Content: Chapter 1 Electrophoresis (pages 9–43): Chapter 2 Isotachophoresis (pages 45–49): Chapter 3 Isoelectric Focusing (pages 51–65): Chapter 4 Blotting (pages 67–80): Chapter 5 Interpretation of Electropherograms (pages 81–90): Chapter 6 Proteome Analysis (pages 91–119): Chapter 7 Instrumentation (pages 121–132): Chapter 8 Method 1: PAGE of Dyes (pages 145–150): Chapter 9 Method 2: Agarose and Immuno Electrophoresis (pages 151–162): Chapter 10 Method 3: Titration Curve Analysis (pages 163–173): Chapter 11 Method 4: Native PAGE in Amphoteric Buffers (pages 175–187): Chapter 12 Method 5: Agarose IEF (pages 189–196): Chapter 13 Method 6: PAGIEF in Rehydrated Gels (pages 197–210): Chapter 14 Method 7: Horizontal SDS?PAGE (pages 211–230): Chapter 15 Method 8: Vertical PAGE (pages 231–245): Chapter 16 Method 9: Semi?Dry Blotting of Proteins (pages 247–255): Chapter 17 Method 10: IEF in Immobilized pH Gradients (pages 257–273): Chapter 18 Method 11: High?Resolution 2?D Electrophoresis (pages 275–298): Chapter 19 Method 12: PAGE of Double Stranded DNA (pages 299–311): Chapter 20 Method 13: Native PAGE of Single Stranded DNA (pages 313–319): Chapter 21 Method 14: Denaturing Gradient Gel Electrophoresis (pages 321–329): Chapter 22 Method 15: Denaturing PAGE of DNA (pages 331–337):