Plant Cell Culture: Essential Methods

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.Content: Chapter 1 Plant Micropropagation (pages 1–23): Ivan Iliev, Alena Gajdosova, Gabriela Libiakova and Shri Mohan JainChapter 2 Thin Cell Layers: The Technique (pages 25–37): Jaime A. Teixeira da Silva and Michio TanakaChapter 3 Plant Regeneration – Somatic Embryogenesis (pages 39–59): Kim E. Nolan and Ray J. RoseChapter 4 Haploid Plants (pages 61–78): Sant S. Bhojwani and Prem K. DantuChapter 5 Embryo Rescue (pages 79–95): Traud Winkelmann, Antje Doil, Sandra Reinhardt and Aloma EwaldChapter 6 In vitro Flowering and Seed Set: Acceleration of Generation Cycles (pages 97–110): Sergio J. Ochatt and Rajbir S. SangwanChapter 7 Induced Mutagenesis in Plants Using Physical and Chemical Agents (pages 111–130): Chikelu Mba, Rownak Afza, Souleymane Bado and Shri Mohan JainChapter 8 Cryopreservation of Plant Germplasm (pages 131–151): E. R. Joachim Keller and Angelika SenulaChapter 9 Plant Protoplasts: Isolation, Culture and Plant Regeneration (pages 153–173): Michael R. Davey, Paul Anthony, Deval Patel and J. Brian PowerChapter 10 Protoplast Fusion Technology – Somatic Hybridization and Cybridization (pages 175–198): Jude W. Grosser, Milica calovic and Eliezer S. LouzadaChapter 11 Genetic Transformation – Agrobacterium (pages 199–215): Ian S. CurtisChapter 12 Genetic Transformation – Biolistics (pages 217–239): Fredy Altpeter and Sukhpreet SandhuChapter 13 Plastid Transformation (pages 241–260): Bridget V. Hogg, Cilia L. C. Lelivelt, Aisling Dunne, Kim?Hong Nguyen and Jacqueline M. NugentChapter 14 Molecular Characterization of Genetically Manipulated Plants (pages 261–279): Cristiano Lacorte, Giovanni Vianna, Francisco J. L. Aragao and Elibio L. RechChapter 15 Bioreactors (pages 281–296): Spiridon KintziosChapter 16 Secondary Products (pages 297–315): Kexuan Tang, Lei Zhang, Junfeng Chen, Ying Xiao, Wansheng Chen and Xiaofen SunChapter 17 Plant Cell Culture – Present and Future (pages 317–331): Jim M. Dunwell